Contents and components: Total-IgE Kit for 96 Tests<\/h2><\/div>\r\nIgE microtiter plate:<\/div><\/div>The pack contains a microtiter plate with 12 strips x 8 wells. Each Well is coated with monoclonal anti-Human IgE (mouse). Store the unused strips together with the desiccant at 2-8\u00b0C in the closeable plastic bag.<\/div><\/div>\r\nAnti-IgE biotin conjugate:<\/div><\/div>The vial contains 13 ml anti-IgE biotene conjugate in Tris-buffer, pH 8.1, with stabilisers and preservatives. Ready to use.<\/div><\/div>\r\nTotal-IgE-calibration:<\/div><\/div>6 vials (0.5 ml each) of human IgE in concentrations 0 – 10 – 50 – 250 – 500 – 1000 kIU\/l (2nd IRP 75\/502 WHO), in horse serum with 0.01% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\nIncubation buffer:<\/div><\/div>The vial contains 18 ml TRIS-buffer, pH 8.4, with 0.1% Tween 20 and 0.005% Proclin 300 as preservative. Ready to use.<\/div><\/div>\r\nSample dilution liquid:<\/div><\/div>The vial contains 10.0 ml of horse serum with 0.01% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\nHRP-Streptavidin-Conjugate:<\/div><\/div>The vial contains 13 ml of streptavidin-peroxidase-conjugate in red colored buffer, pH 5.5, with 0.001% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\nSubstrate HS:<\/div><\/div>The vial contains 13 ml of a stabilized mixture of 3.3′, 5.5′ thetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Ready to use.<\/div><\/div>\r\nStopping Liquid:<\/div><\/div>The vial contains 13 ml of 0.3 M of sulphuric acid. Ready to use.<\/div><\/div>\r\nWashing buffer concentrate 10x:<\/div><\/div>The vial contains 100 ml of a 10x concentrated washing liquid, with 2.5 \u03bcg of amphotericin B. To prepare the working liquid, mix the contents of the vial with 900 ml of distilled water.<\/div><\/div>\r\n<\/article>","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-4276","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/comments?post=4276"}],"version-history":[{"count":17,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276\/revisions"}],"predecessor-version":[{"id":5702,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276\/revisions\/5702"}],"wp:attachment":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/media?parent=4276"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
IgE microtiter plate:<\/div>
<\/div>
The pack contains a microtiter plate with 12 strips x 8 wells. Each Well is coated with monoclonal anti-Human IgE (mouse). Store the unused strips together with the desiccant at 2-8\u00b0C in the closeable plastic bag.<\/div><\/div>\r\n
Anti-IgE biotin conjugate:<\/div>
<\/div>
The vial contains 13 ml anti-IgE biotene conjugate in Tris-buffer, pH 8.1, with stabilisers and preservatives. Ready to use.<\/div><\/div>\r\n
Total-IgE-calibration:<\/div>
<\/div>
6 vials (0.5 ml each) of human IgE in concentrations 0 – 10 – 50 – 250 – 500 – 1000 kIU\/l (2nd IRP 75\/502 WHO), in horse serum with 0.01% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\n
Incubation buffer:<\/div>
<\/div>
The vial contains 18 ml TRIS-buffer, pH 8.4, with 0.1% Tween 20 and 0.005% Proclin 300 as preservative. Ready to use.<\/div><\/div>\r\n
Sample dilution liquid:<\/div>
<\/div>
The vial contains 10.0 ml of horse serum with 0.01% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\n
HRP-Streptavidin-Conjugate:<\/div>
<\/div>
The vial contains 13 ml of streptavidin-peroxidase-conjugate in red colored buffer, pH 5.5, with 0.001% Proclin 300 as a preservative. Ready to use.<\/div><\/div>\r\n
Substrate HS:<\/div>
<\/div>
The vial contains 13 ml of a stabilized mixture of 3.3′, 5.5′ thetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Ready to use.<\/div><\/div>\r\n
Stopping Liquid:<\/div>
<\/div>
The vial contains 13 ml of 0.3 M of sulphuric acid. Ready to use.<\/div><\/div>\r\n
Washing buffer concentrate 10x:<\/div>
<\/div>
The vial contains 100 ml of a 10x concentrated washing liquid, with 2.5 \u03bcg of amphotericin B. To prepare the working liquid, mix the contents of the vial with 900 ml of distilled water.<\/div><\/div>\r\n<\/article>","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-4276","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/comments?post=4276"}],"version-history":[{"count":17,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276\/revisions"}],"predecessor-version":[{"id":5702,"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/pages\/4276\/revisions\/5702"}],"wp:attachment":[{"href":"https:\/\/www.radim.com\/en\/wp-json\/wp\/v2\/media?parent=4276"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}