Radim Diagnostics

Alisei Q.S.

Fully automatic microtiter plates ELISA Analyzer

Capacity of 6 microtiter plates (ELISA)
Walk-Away-System
Ideal for various ELISA applications of laboratory diagnostics:
Determination of hormones, tumor markers, infectious diseases …
Up to 12 different assays at same time
Standard curve recall and recalibration
Time management system
Quality control functions
Monitoring the entire test run
No disposable tips
Flexible user interface

Solution for processing ELISA kits

RADIM Germany, in collaboration with an Italian manufacturer of diagnostic instruments, offers you a solution for the automated application of ELISA assays – the automated ELISA instrument Alisei Q.S.. Our kits are for use on Alisei Q.S. designed and the test protocols are preinstalled.

Simple ‘loading’ with reagent and sample racks, which can be stored in the refrigerator.

6 microtiter plates (ELISA) are incubated, shaken and washed independently of each other

Reagents and samples

Removable rotating racks for reagents and sample tubes
Maximum capacity: 240 sample tubes
Simultaneous loading of up to 12 reagent sets (or up to 81 allergens)

The Alisei Q.S. uses bare-coded reagents to ensure maximum control during testing, and a validated test routine from the assay. All bottles (reagents – standards – controls) are housed on chilled plates.
Multiple reagents on two separate racks can be loaded on the machine. The charging area is easily accessible for easy loading of reagents, samples and polymer tips through a transparent, large sliding window. The reagent and sample racks are easily removable with practical handles to quickly store the reagents in the refrigerator.

Double pipetting system

The pipetting system consists of two independent needles that are able to aspirate from two adjacent samples or from the same reagent and dispense into two separate wells simultaneously. Each needle is connected to two syringe pumps (2500 µL and 1000 µL) to dispense reagent or sample. To ensure the correct functioning of the system, each needle is equipped with a fluid and clot detection system.

two independent needles

Barcode reader offers reliability

The sample identification allows true positive sample identification of samples (PSID) via an integrated barcode reader. This new feature results in the most reliable sample collection, sample identification and match test results and prevents user error.

Barcode reader reads samples and reagents

Dosing and diluting

The Alisei Q.S. has a high-precision robotic arm powered by stepper motors and controlled by optical and mechanical sensors. It is equipped with 2 dosing systems / needles, which make it possible to work independently of each other and to enable highly efficient pipetting.

Pipettier speed serum: 700 samples/hour
Pipettier speed reagents: 1500 Wells/Hour
Liquid level detection for optimal detection of fibrin clots and insufficient volume

Incubate and mixing

The microtiter plate carousel is designed to process 6 microtiter plates at the same time. Each microtiter plate can be edited and programmed completely individually. In addition, a single microtiter plate can be run.
The microtiter plate carousel can perform linear shaking with a programmable intensity and period for each plate separately.

8-channel washing station

The washing station has an 8-channel wash head with two parallel needles each, suitable for washing both ‘U’ and flat bottom wells. Cycles, volume and number of washes can be easily programmed by the operator and three washing buffers + distilled water can be loaded on board at the same time.

Washing volume: programmable from 100 to 350 µl
Number of cycles: programmable from 1 to 9 cycles
Impact time: programmable from 1 second to 3 minutes
Pressure: programmable

Each protocol can be programmed by the operator with multiple washes that differ in cycle, volume, holding time, and pressure.

Alisei Q.S.: 8-channel washing station

Reading

The reading station has an 8-channel photometer, which can be programmed by the operator for reading with a single, double or triple beam (vertical measurement). With a triple beam (over-range measurement), the instrument automatically converts the absorbance values measured at the different wavelengths. Measurements can be taken at one, two or more wavelengths.

The normally provided filters: 405, 450, 492, 550 and 620 nm
Reader range: 0-3 OD in Bichromatism; 0-9 OD in polychromatism

Alisei Q.S.: 8-channel photometer

Equipment maintenance

The Alisei Q.S. has been a sophisticated, reliable tool for everyday use for years to maximize the efficiency of your laboratory:
Preparation and follow-up of working time: 5 minutes per day
No warm-up phase required.
Self-diagnostic and alarm procedures are an essential part of the Alisei Q.S..

Simple maintenance procedures:

daily

Cleaning at the end of the run – fully automatic.

weekly

General cleaning of the workspace of liquids.
Empty all tanks to remove residual buffers. Rinse with DI water.

monthly

Wipe off the work area, plate trays, and waste bin.
Check tanks and visible hoses

Smart software

Intelligent software control allows you to quickly, easily customize, monitor and manage all settings and data, as well as process and deliver results to your lab information system:

Programming and changing test protocols
Insert work lists with all relevant patient data
Monitoring the entire test run
View and manage the final results
Personalizing and printing the patient report
Standard curve retrieval function
T. M. S. (Time Management System)
Quality control functions
Compatible with laboratory information systems

ELISA results manager

The software supports the processing of raw data from ELISA tests and their accurate conversion to the final concentration units. It allows evaluation of the test results using the most appropriate calculation models for the ELISA signal-concentration relationship.
Logistic curve-based parametric methods such as 4-parameter logistics (4PL) and Live Parameter Logistic (5PL) models have the closest correlation with real signal concentration dependence in ELISA assays.

The calculated results based on these methods have very little variability and high reproducibility. Therefore, they are considered the most suitable models for both competitive and “sandwich” ELISAs viewed. However, these calculation modes are not always available in the standard equipment software for demonstrating the ELISA test results. In this case, the ELISA Results Manager software provides an upgrade of the results analysis.

Key program benefits:

Calculating results based on 4PL and 5PL models
Visualization of the calibration curves
Simplified information entry
Processing the data received on an ELISA device
Calculation of sample concentrations and statistics
Printable results

Technical specifications Alisei Q.S.

Dosing

microtiter plates:

Parameter:

max. 12 in one run

Samples:

max. 360 in one run

Pipetting volume:

7 µL – 1000 µL

Precision tip 10 µL:

less than 2.5%

Tipp 100 µL:

less than 1%

Needle:

less than 1.5%

Throughput samples:

700 / h (500 µL washing)

Throughput reagents:

1400 Wells / h

Fill level sensor:

Capazitive for needle

Incubators:

Temperature:

RT / RT +5 °C bis +45 °C

Heating time:

5 min

Washing

Buffer:

4 with filler stander recognition

Stations:

Liquid trap:

10L with “Full” sensor

Photometer

Units:

Reading:

from 0-3 OD (mono / dual / triple wavelength)

Spectral range:

400 – 700 nm

Interference filters:

Software

Operating system:

Windows 10

Interface:

Bidirectional ASTM (LIS compatible)

Internal quality control:

Levey Jennings and Westguard rules

Data reduction:

Standard interpolations

Dimensions

Depth:

73 cm

Height:

60 cm

Width:

140 cm

Weight:

100 kg

Electrical specifications

Voltage:

110 or 220 V AC

Frequency:

50-60 Hz

Power:

800 VA

Compliance

Safety CE certified